Discovery of green fluorescent protein / Osamu Shimomura -- Photons for reporting molecular events: green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin -- ...
Fluorescent protein chromophores are the light‐emitting centres intrinsic to proteins such as green fluorescent protein (GFP) and its variants. These chromophores emerge through post‐translational ...
Fluorescent proteins (FPs) have transformed biological imaging by enabling detailed visualisation of cellular and molecular processes in real time. Initially exemplified by the green fluorescent ...
Modern bioassays tend to fall under three common modalities: colorimetric, luminometric or fluorometric. Colorimetric assays record the amount of light absorbed by a reporter compound. This absorbance ...
It has been over 60 years since Osamu Shimomura et al. discovered Green Fluorescent Protein (GFP) 1. Since then, the color palette for fluorescent proteins has been extended to span blue through to ...
Following chemist and marine biologist Osamu Shimomura’s success in determining the structure of luciferin from a crustacean at Nagoya University, Frank Johnson, then a biologist at Princeton ...
Fruit flies, mice, zebrafish, yeast and the tiny worm C. elegans are model organisms that have carried modern biology on their backs. Scientists did not choose them for their charisma. They were ...