Dye-ligand Affinity Chromatography for Protein Purification
Dye-ligand Affinity Chromatography for Protein Purification
Oct 28, 2018
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SOLVED: I need help on calculating the concentration. A protein assay requires the reaction of the protein solution with a protein-binding dye for one minute followed by measuring the absorbance of the protein-dye complex at 505 nm. The absorbance data for four protein solutions and the blank is provided in the table below. The absorbance of each solution was measured three times. Construct a calibration curve using the data in the table, and find the slope (m), intercept (b), and their respecti
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